610nm optical filter for PCR fluorescence detection filter

  • PCR Fluorescence Sensor - opticalfiltershop.com

    PIXELTEQ's PCR Sensor is a ready-to-use compact sensor specifically tailored for detecting common fluorescence in PCR reactions. In this 9x9mm array we have packed the capability to detect 4 different fluorescence signals simultaneously in one optical path.

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  • 610nm CWL 12.5mm Dia. Hard Coated OD 4 10nm Bandpass Filter

    610nm CWL 12.5mm Dia. Hard Coated OD 4 10nm Bandpass Filter ... fluorescence laser diode cleanup or chemical detection or analysis. TECHSPEC Hard Coated OD 4 10nm Bandpass Filters feature durable hard coatings to minimize filter degradation while increasing transmission. ... These optical filters offer steep slopes with deep blocking to ...

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  • Custom Optical Filters Fluorescence Filter Cubes ...

    Custom-designed optics including optical filters and filter cubes for imaging at the desired resolution on cellular tissue and in vivo imaging systems.

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  • Selecting Optical Filters for Fluorescence Microscopy—Note ...

    Sensitive and versatile fluorescence detection techniques are of ever-increasing importance and popularity in biological research microscopy. In the now-standard epi-illuminated microscope configuration the optical filter set performs a critical function in separating the fluorescence emission photons that will form the final image from the more-intense excitation light field.

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  • Aria Real-Time PCR System Optical Cartridges Agilent

    The Aria Real-Time PCR System is a fully integrated quantitative PCR amplification detection and data analysis system. The system combines a state-of-the-art thermal cycler an advanced optical system with an LED excitation source and complete data analysis software. The Aria System can be used with many fluorescence detection chemistries including SYBR Green EvaGreen dyes and probes.

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  • Filters Fluorescence and Life Science Instrumentation

    Omega Optical celebrates 40 years! 2 omega optical has been designing and producing optical filters for fluorescence microscopy and instrumentation longer than any other optical filter company. We are grateful for the advancements in science technology and outer space made with our

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  • Real Time PCR - physlab.org

    detection. Normal PCR. PCR (Polymerase Chain Reaction) ... (camera photo detector connected to optical filters) to capture the fluorescence emitted of the probe. Basic Set-up (continued) • A computer to regulate the heating cycle and record the data from the receiver

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  • CFX384 Touch Real-Time PCR Detection System

    The CFX384 Touch Real-Time PCR Detection System is a powerful flexible ... The optical filter sets are designed to maximize fluorescence detection for specific dyes in specific channels. At every position and with every scan the optics shuttle is reproducibly centered above each ...

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  • Fluorescence Imaging Filters - Thorlabs

    These excitation emission and dichroic filters are designed specifically for use in fluorescence imaging applications. They are fabricated at industry-standard dimensions that make them compatible with filter cubes from all major manufacturers. We offer individual filters and filter sets targeted

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  • Introduction to Fluorescence Filters - Semrock

    Semrock manufactures hundreds of standard optical filters for immediate shipping: bandpass filters dichroic beamsplitters edge and notch filters Raman spectroscopy filters mirrors and fluorescence filters sets. For Custom optical filters engineering design or system design support please contact us to discuss your specific needs.

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  • 610nm CWL 12.5mm Dia. Hard Coated OD 4 10nm Bandpass Filter

    610nm CWL 12.5mm Dia. Hard Coated OD 4 10nm Bandpass Filter ... fluorescence laser diode cleanup or chemical detection or analysis. TECHSPEC Hard Coated OD 4 10nm Bandpass Filters feature durable hard coatings to minimize filter degradation while increasing transmission. ... These optical filters offer steep slopes with deep blocking to ...

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  • Filters Fluorescence and Life Science Instrumentation

    Omega Optical celebrates 40 years! 2 omega optical has been designing and producing optical filters for fluorescence microscopy and instrumentation longer than any other optical filter company. We are grateful for the advancements in science technology and outer space made with our

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  • Aria Real-Time PCR System Optical Cartridges Agilent

    The Aria Real-Time PCR System is a fully integrated quantitative PCR amplification detection and data analysis system. The system combines a state-of-the-art thermal cycler an advanced optical system with an LED excitation source and complete data analysis software. The Aria System can be used with many fluorescence detection chemistries including SYBR Green EvaGreen dyes and probes.

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  • Smartphone detection of antibiotic resistance using ...

    The efficient choice of the fluorescence filters allowed simultaneous detection of 565 nm and 625 nm fluorescence from two different quantum dots. The fluorescence reader also allows detection of 655 nm 585 nm and 605 nm emission quantum dots (Fig. S3) to allow simultaneous detection of more than two targets.

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  • CFX384 Touch Real-Time PCR Detection System

    The CFX384 Touch Real-Time PCR Detection System is a powerful flexible ... The optical filter sets are designed to maximize fluorescence detection for specific dyes in specific channels. At every position and with every scan the optics shuttle is reproducibly centered above each ...

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  • Fluorescence Microscopy - the Magic of Fluorophores and ...

    Fluorescence Microscopy – the Magic of Fluorophores and Filters ... Knowing the excitation and emission spectra of a fluorophore is critical when choosing optimal light sources and filters for your fluorescence microscope as well as when visualizing more than one fluorophore in a sample. ... check out Bitesize Bio's guide to buying a ...

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  • CFX96 Touch Real-Time PCR Detection System

    The optical filter sets are designed to maximize fluorescence detection for specific dyes in specific channels. At every position and with every scan ... Touch™ Real-Time PCR Detection System to genotype samples based on their DNA thermal denaturation properties. The software can be used for a

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  • Fluorescence Imaging Filters - Thorlabs

    These excitation emission and dichroic filters are designed specifically for use in fluorescence imaging applications. They are fabricated at industry-standard dimensions that make them compatible with filter cubes from all major manufacturers. We offer individual filters and filter sets targeted

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  • Fluorescence Fundamentals Thermo Fisher Scientific - US

    Four essential elements of fluorescence detection systems can be identified from the preceding discussion: 1) an excitation light source (Figure 5) 2) a fluorophore 3) wavelength filters to isolate emission photons from excitation photons (Figure 5) 4) a detector that registers emission photons and produces a recordable output usually as an ...

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  • Chroma Technology Optical Filters Custom & OEM Filter ...

    Chroma Technology designs and manufactures optical filters: thin film coated bandpass notch edge and laser filters dichroic beamsplitters and mirrors. Chroma Technology Optical Filters Custom & OEM Filter Design Imaging Systems & Products

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  • LightCycler 480 Detection Formats - Roche

    LightCycler 480 Detection Formats Due to its highly flexible selection of excitation and emission wavelengths the LightCycler 480 Real-Time PCR System is compatible with most currently available dyes and assay formats yet is also adaptable to new future applications.

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  • Real-time PCR System - Techne

    Fluorescence excitation range 470-650nm (standard filters are centred at 485 530 580 and 640nm) Fluorescence detection range 500-710nm (standard filters are centred at 520 560 615 and 685nm) Dynamic range At least 9 orders of magnitude of target DNA concentration Sensitivity (detection threshold) 1nM fluorescein in a 20ul sample

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  • Development of a CCD-based fluorimeter for real-time PCR ...

    This fluorescence detection system consists of a 470 nm light emitting diode (LED) as an excitation light source a CCD camera and a rotating disk with six optical filters to provide six fluorescence detection channels at 530 560 610 640 670 and 710 nm for multiplex real-time PCR DNA quantification.

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  • PCR device with integrated thermal cycling and ...

    In this paper we present both a theoretical evaluation and the fabrication of a novel polymerase chain reaction (PCR) microdevice. This microdevice contains elements for both thermal cycling and fluorescence detection. The proposed device is composed of a reaction chamber with integrated temperature sensor heaters p-n diode and optical filter.

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  • Filter Emission 610nm LP PerkinElmer

    PerkinElmer Filter Emission 610nm LP - 131253. A long history in transplant medicine and now the era of Regenerative Medicine.

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  • Agilent AriaMx Real-Time PCR System Agilent

    Agilent AriaMx is a fully integrated qPCR solution for amplification detection and data analysis combining a thermal cycler an optical system with LED excitation source and data analysis software. AriaMx can be used with many fluorescence detection chemistries including SYBR Green EvaGreen and …

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  • Eco Real-Time PCR System - Illumina

    Figure 2: Eco Real-Time PCR System Innovative Design Features The Eco Real-Time PCR system offers the qPCR capabilities of larger instruments in a compact footprint. Innovative features include a precise thermal system for unrivaled temperature control an advanced optical system for highly sensitive fluoresence detection a 48-well plate for

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  • virotype PRRSV RT-PCR Kit Handbook

    fluorescence intensities during the PCR run (i.e. in real time) allows the detection of the accumulating product without the need to re-open the reaction tubes afterward. The virotype PRRSV RT-PCR Kit contains all of the necessary reagents for the detection of PRRSV RNA including a positive

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  • Biological detection Kategoriat Environics Oy

    Biological detection. ... In the optical system the fluorescence light emitted and the light scattered by the particles in the air flow is gathered and directed with mirrors and optical filters to photomultiplier tubes that record the scattering and fluorescence signals. This signal information is processed by the advanced alarm algorithm to ...

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  • FIBER OPTIC MICRO-ENDOSCOPY FOR DETECTION OF …

    Fiber Optic Micro-endoscopy for Detection of Bacteria in Early Stages of Infection. (December 2010) ... techniques such as polymerase chain reaction (PCR) and the enzyme linked immuno- ... but not in real time or in vivo. Optical imaging and detection utilizes light to interrogate a sample and detects

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  • CFX Connect Real-Time PCR Detection System

    The CFX Connect Real-Time PCR Detection System offers two-target analysis ... The optical filter sets are designed to maximize fluorescence detection for specific dyes in specific channels. At every position and with every scan the optics shuttle is reproducibly ...

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  • Optics of a Flow Cytometer Thermo Fisher Scientific - US

    Optics of a Flow Cytometer. ... If you are not familiar with basic fluorescence concepts or with filters and light sources in general we highly recommend that you review the Molecular Probes School of Fluorescence—Fluorescence Basics ... The components of the optical system work in concert to shine different wavelengths of light onto the ...

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  • PCR device with integrated thermal cycling and ...

    In this paper we present both a theoretical evaluation and the fabrication of a novel polymerase chain reaction (PCR) microdevice. This microdevice contains elements for both thermal cycling and fluorescence detection. The proposed device is composed of a reaction chamber with integrated temperature sensor heaters p-n diode and optical filter.

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  • Fluorescence Fundamentals Thermo Fisher Scientific - US

    Four essential elements of fluorescence detection systems can be identified from the preceding discussion: 1) an excitation light source (Figure 5) 2) a fluorophore 3) wavelength filters to isolate emission photons from excitation photons (Figure 5) 4) a detector that registers emission photons and produces a recordable output usually as an ...

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  • Fluorescence detection in Lab-on-a-chip systems using ...

    Fluorescence detection in Lab-on-a-chip systems using ultrafast nucleic acid amplification methods Rainer Gransee1 Tristan Schneider2 Deniz Elyorgun3 Xenia Strobach1 Tobias Schunck1 Theresia ...

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  • LightCycler 480 Detection Formats - Roche

    LightCycler 480 Detection Formats Due to its highly flexible selection of excitation and emission wavelengths the LightCycler 480 Real-Time PCR System is compatible with most currently available dyes and assay formats yet is also adaptable to new future applications.

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  • qPCR for who you are - Thermo Fisher Scientific

    qPCR for who you are QuantStudio real-time PCR and digital PCR systems. Every lab is unique. That's why you deserve a qPCR ... (21 filter combinations) 5 colors 6 colors (21 filter combinations) 6 colors (21 filter combinations) ... Fluorescence melt curve plot and derivative curve plot for …

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  • Effect of a high surface-to-volume ratio on fluorescence ...

    In the work discussed in this paper the effect of a high surface-to-volume ratio of a microfluidic detection cell on fluorescence quenching was studied. It was found that modification of the geometry of a microchannel can provide a wider linear range. This is …

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  • Simplify your PCR fluorescence detection

    Simplified Multiplexed Fluorescence Detection. Monitor up to 4 markers simultaneously with one detector array! Learn More

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  • Fluorescence micro-optics Fluorescence detectors

    Features of NSG's Fluorescence micro-optics - 1. Small optical probes for parallel detection. Small optical probes composed of SELFOC microlenses and fibers can. be placed close to specimen or can be applied to realize array heads. No more scan … No more 2-D image sensors … A 16ch probe array of 4.5mm-pitch for 384ch micro-well plates.

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  • virotype BTV pan/8 RT- PCR Kit Handbook

    fluorescence intensities during the PCR run (i.e. in real time) allows the detection of the accumulating product without the need to re-open the reaction tubes afterward. The virotype BTV pan/8 RT-PCR Kit contains all of the necessary reagents for the detection of …

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  • CFX96 Real-Time PCR Detection System

    The CFX96 real-time PCR detection system builds on the power and flexibility of the C1000 ... The optical filter sets are designed to maximize fluorescence detection for specific dyes ... Linearity of five-target multiplex detection. A–E fluorescence data from a series …

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  • The LightCycler 480 System Unleash the Potential of Real ...

    Optical path PCR multiwell plate CCD camera Emission filter wheel Excitation filter wheel Table 1: Overview of excitation and emission filters dyes and detection formats. The LightCycler 480 Instrument employs the high-intensity LightCycler 480 Xenon Lamp that emits light over a broad wavelength range (430–630 nm). The

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  • Critical assessment of digital PCR for the detection and ...

    Polymerase chain reaction (PCR) is the leading DNA-based method for GMO detection. ... as all platforms include filters that allow detection of fluorescence in at least two channels (FAM and HEX/VIC). ... The choice of the reporters for TaqMan probes is limited by the availability of only a few fluorescence detection channels (usually FAM and ...

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  • PCR device with integrated thermal cycling and ...

    In this paper we present both a theoretical evaluation and the fabrication of a novel polymerase chain reaction (PCR) microdevice. This microdevice contains elements for both thermal cycling and fluorescence detection. The proposed device is composed of a reaction chamber with integrated temperature sensor heaters p-n diode and optical filter.

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  • Four-Channel Multiplexing System

    The fluorescence of up to four different probes can be detected in a single tube. By exciting each dye at its peak wavelength ... Optical Denaturation™ is a patented method that ... Detection Filters: 510nm 555nm 610nm bandpass 660nm 580nm 610nm high-pass ...

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  • Fluorescence Imaging - PiratePanel

    PCR product analysis microarray analysis and DNA sequencing can all benefit from the application of fluorescence detection. Fluorescence detection offers a number of important advantages over other methods several of which are described below. Sensitivity Fluorescent probes permit sensitive detection of many biological molecules.

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  • Quantitative PCR & Digital PCR Detection Methods - PCR ...

    Quantitative PCR and Digital PCR Detection Methods. A Technical Guide to PCR Technologies ... select the reporter dyes that are compatible with the detection channels for the instrument and ensure that the correct filters and calibration are in place. ... P.M. Abramson R.D. Watson R. et al. Detection of specific polymerase chain reaction ...

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  • Custom Dye Calibration of Applied Biosystems Real Time PCR ...

    spectral profiles plotted as fluorescence versus filter. The pass criteria for a dye calibration is that the dye spectra peak within the same filter as their group. Compatible instruments The procedures in this guide are compatible with the following interchangeable-block real-time PCR instruments or systems. These procedures replace the custom dye

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  • Optimization of flow cytometric detection and cell sorting ...

    Optimization of flow cytometric detection and cell sorting of transgenic Plasmodium parasites using interchangeable optical filters. Ivan A Vorobjev 1 2 ... Based on recent advances in flow cytometer optical filter ... Optimization of optical filters allowed for the detection of 50-150% more GFP-expressing parasites as compared to use of ...

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  • OSA Highly sensitive lens-free fluorescence imaging ...

    We report a lens-free fluorescence imaging device using a composite filter composed of an interference filter and an absorption filter each applied to one side of a fiber optic plate (FOP). The transmission of angled excitation light through the interference filter is absorbed by the absorption filter. The auto-fluorescence of the absorption filter is reduced by the reflection from the ...

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  • Miniature real time PCR on chip with multi ... - SpringerLink

    This paper presents the design and implementation of a miniature real time PCR system consisting of a disposable reactor chip a miniature thermal cycler and a multi-channel fiber optical fluorescence excitation/detection module. The disposable PCR chip is fabricated by using soft photolithography by PDMS (Polydimethylsiloxane) and glass.

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  • Development of a CCD-based fluorimeter for real-time PCR ...

    This fluorescence detection system consists of a 470 nm light emitting diode (LED) as an excitation light source a CCD camera and a rotating disk with six optical filters to provide six fluorescence detection channels at 530 560 610 640 670 and 710 nm for multiplex real-time PCR …

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  • Supporting Information for: Visible Fluorescence Detection ...

    Visible Fluorescence Detection of Type III Protein Secretion ... The SopE2 promoter region and coding sequence was amplified by PCR from S. typhimurium strain IR715 a ... 488 nm and the emission was collected through a BP 505-550 filter. TOPRO-3 was excited with a HeNe laser at 633 nm and the emission was collected through a LP 650 ...

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  • Fluorescence micro-optics Fluorescence detectors

    Features of NSG's Fluorescence micro-optics - 1. Small optical probes for parallel detection. Small optical probes composed of SELFOC microlenses and fibers can. be placed close to specimen or can be applied to realize array heads. No more scan … No more 2-D image sensors … A 16ch probe array of 4.5mm-pitch for 384ch micro-well plates.

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  • Product Quantity on hand ANALYTICAL INSTRUMENTS AND …

    Product Quantity on hand ANALYTICAL INSTRUMENTS AND EQUIPMENT Autoclave Autoclave Benchtop Market Forge SIM-E 1 ... Filter >610nm cs 29 em:chlorophyll Turner Designs 10- ... Filter Optical 2 Part of Optical Kit-Chlorophyll Lab extractive Measurement Turner Designs 10-040R 1 ...

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  • Optimization of flow cytometric detection and cell sorting ...

    Optimization of flow cytometric detection and cell sorting of transgenic Plasmodium parasites using interchangeable optical filters. Ivan A Vorobjev 1 2 ... Based on recent advances in flow cytometer optical filter ... Optimization of optical filters allowed for the detection of 50-150% more GFP-expressing parasites as compared to use of ...

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  • qPCR Workshop held at NFSTC Peter Vallone (NIST) and ...

    Real-Time PCR Instrumentation As the amount of amplified DNA in the PCR increases there is a change in the amount of fluorescence Organic dyes – Free in solution (SYBR Green I) – Attached to a probe FAM S N+ N N N SYBR Green I Zipper et al. Nucleic Acids Research 2004 32: e103 Fluorescence Detection

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  • Plasmonics Enhanced Smartphone Fluorescence Microscopy ...

    Smartphone fluorescence microscopy has various applications in point-of-care (POC) testing and diagnostics ranging from e.g. quantification of immunoassays detection …

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  • OPEN Plasmonics Enhanced Smartphone Fluorescence …

    Fluorescence Microscopy Qingshan Wei1 2 3 4 Guillermo Acuna5 6 7 Seungkyeum Kim8 ... the number of target molecules before the optical detection step the development of higher sensitivity mobile ... excluding the optical filter. Optimization of the substrate structure for SEF.

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  • 510(k) SUBSTANTIAL EQUIVALENCE DETERMINATION …

    images correspond to the 96 reaction wells of the PCR plate. • Background fluorescence data indicating the signal from each well with no fluorescent dye present. • Pure dye spectral data indicating the relative signal intensity for the five optical filter channels when fluorescence of a single pure dye is measured.

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  • Combined thioflavin T–Congo red fluorescence assay for ...

    Fluorescence represents one of the most powerful tools for the detection and structural characterization of the pathogenic protein aggregates amyloid fibrils. The traditional approaches to the identification and quantification of amyloid fibrils are based on monitoring the fluorescence

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  • The LightCycler 480 System - Unleash the Potential of ...

    optical path PCR multiwell plate CCD camera emission filter wheel excitation filter wheel detection formats. The LightCycler 480 Instrument employs a high-intensity LED that emits light over a broad wavelength range (390–710 nm). The five excitation and six emission filters of the instrument can be used in any combination.

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  • Rainbow Vectors for Broad-Range Bacterial Fluorescence ...

    Fluorescent proteins were amplified by polymerase chain reaction (PCR) ... Flow cytometry can be used for cell detection using fluorescence labeling. This method can also be used for the detection of free bacteria as well as bacteria bound to eukaryotic cells. ... coli reinforce the importance of using the right filter for fluorescence imaging ...

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  • MODULE 5: SINGLE MOLECULE FLUORESCENCE

    detector. A tube lens creates the fluorescence image on the CCD. Additionally since the excitation laser may be orders of magnitude brighter than the single molecule fluorescence you will observe a longpass filter†† (see Figure 6) rejects any laser light that happens to leak through the dichroic.

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  • Custom Dye Calibration of Applied Biosystems Real Time PCR ...

    spectral profiles plotted as fluorescence versus filter. The pass criteria for a dye calibration is that the dye spectra peak within the same filter as their group. Compatible instruments The procedures in this guide are compatible with the following interchangeable-block real-time PCR instruments or systems. These procedures replace the custom dye

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  • Thermo Scientific ABgene Thermo-Fast 96 PCR Detection ...

    Shop online for a wide selection of Thermo Scientific ABgene Thermo-Fast 96 PCR Detection Plate MKIII 96-well low-profile semi-skirted PCR plate for the fast block of Life

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  • IntelliQube Detection Douglas Scientific

    IntelliQube Detection . The IntelliQube features an advanced optical system for inline fluorescence detection in Array Tape .Filtered LEDs are optimized for a variety of commonly used fluorophores with the excitation range of 480-620 nm.

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  • Critical assessment of digital PCR for the detection and ...

    Polymerase chain reaction (PCR) is the leading DNA-based method for GMO detection. ... as all platforms include filters that allow detection of fluorescence in at least two channels (FAM and HEX/VIC). ... The choice of the reporters for TaqMan probes is limited by the availability of only a few fluorescence detection channels (usually FAM and ...

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  • CT&NG Real Time PCR Kit User Manual For In Vitro ...

    CT&NG Real Time PCR Kit User Manual For In Vitro ... Fluorescence is emitted and measured by the real time systems´ optical unit during PCR. The detection of amplified Chlamydia trachomatis DNA fragment is performed in fluorimeter channel 530nm with the fluorescent quencher BHQ1. ... the kit contains a system to identify possible PCR ...

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  • PCR Detection of Bacteria in Seven Minutes Science

    Detection profiles obtained by rapid real-time PCR analysis of Erwinia cells. (A) Effect of decreasing the cycle time for the analysis of 500 cells. The number to the right of each detection profile is the cycle time listed in Table 1. (B) Quantitative PCR performed with the 17-s cycle time. The number of bacteria cells and the time that a ...

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  • High Resolution Melting: History Technology and Utility

    High Resolution Melting: History Technology and Utility Charles Hardwick Ph.D Field Applications Consultant ... – high sensitivity optical system (light source filters and detection system) ... fluorescence detection Filter wheels CCD Camera Multiwell plate

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  • Fluorescence Microscope- Principle Instrumentation ...

    Fluorescence Microscope- Principle Instrumentation Applications Advantages Limitations. A fluorescence microscope is an optical microscope that uses fluorescence and phosphorescence instead of or in addition to reflection and absorption to study properties of organic or inorganic substances.

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  • Paper-based RNA detection and multiplexed analysis for ...

    Experiments in Guinea. (a) Picture of the transportable equipment: heating device and optical system of detection fixed in a suitcase.(b) Amplification curve for a negative patient sample (green ...

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  • filter Sigma-Aldrich

    Summary: Normal blood lactate is maintained at about 1.5 mM and little filtered lactate is excreted in urine. Reabsorption of lactate is mediated by the low-affinity Na(+)-coupled lactate transporter SLC5A12 in the initial part of the proximal tubule and by the high-affinity Na(+)-coupled lactate transporter SLC5A8 (MIM 608044) in the distal proximal tubule (Gopal et al. 2007 [PubMed 17692818]

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  • LightCycler 480 Instrument II - Roche

    The LightCycler 480 System is a high-performance medium- to high-throughput PCR platform (96- or 384-well plates) that provides various methods for gene detection gene expression analysis genetic variation analysis and array data validation. The system features the LightCycler 480 Instrument a versatile plate-based real-time PCR device that supports mono- or multicolor applications ...

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  • virotype BTV pan/8 RT- PCR Kit Handbook

    fluorescence intensities during the PCR run (i.e. in real time) allows the detection of the accumulating product without the need to re-open the reaction tubes afterward. The virotype BTV pan/8 RT-PCR Kit contains all of the necessary reagents for the detection of …

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  • artus LC Protocol Sheet

    The background phase lasts until the fluorescence signal from the PCR product exceeds the ... The optical filters of the LightCycler 2.0 have a band width of 20 nm to 40 nm around the specified ... one PCR for the detection of the pathogen and another for the detection of the internal control (IC). Since both systems contain different ...

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  • Knight Optical IR Optics Photonics Lasers Precision ...

    Knight Optical's MD Colin Overton has built a company with global recognition for quality optics components for imaging photonics technology and optical research.

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  • Microplate Readers Fisher Scientific

    RNAi Oligos Assays Gene Editing & Gene Synthesis Tools Oligos Tools. Eurofins MWG Operon Oligos Tool

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  • Applied Biosystems 7300 Real-Time PCR System

    7300 Real-Time PCR System combines thermal cycling fluorescence detection and application-specific software to measure the cycle-by-cycle accumulation of PCR products in a single-tube homogeneous reaction. Quantitative results are available immediately upon completion of PCR with no need to run gels purify PCR products or perform any post-PCR

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  • OEM Capability - Your Optical Filters Partner for ...

    This is an indication about the filters specification we could produce. It doesnt mean the standard is the same as the specification listed above. Optolong welcomes any OEM requirement about optical filter interference filter fluorescence filters astronomical filter.

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  • LightCycler 480 Real-Time PCR System Rapid by Nature ...

    LightCycler 480 Real-Time PCR System Rapid by Nature – Accurate by Design ... Highest flexibility with fluorescence dyes and detection formats. Availability of increased research time by the ... combine various optical filters and benefit from modular software design.

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  • Integrated wavelength-selective optical waveguides for ...

    Through the detection of a BK virus polymerase chain reaction (PCR) product we demonstrate that the dye-doped PDMS system is an order of magnitude more sensitive than a similar undoped system (SNR: 138 vs. 9) without the use of any external optical filters at the detector.

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  • ABI Prism 7000 System and Applied Biosystems 7500 System ...

    ABI PRISM™ 7000 Sequence Detection System and Applied Biosystems 7500 Real-Time PCR System 6 User Bulletin How to View Component Dye Signals To view the component dye signals from a Quantifiler™Kit run select the Component sub-tab within the Results view of the SDS Software.The halogen lamp may need replacement if the lines

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  • Eco Real-Time PCR System User Guide - Illumina

    Eco™ Real-Time PCR System User Guide. ... along with four emission filters and a CCD camera for detection enabling multiplexing of up to four targets. Each instrument comes factory- ... fluorescence approaches the level of optical detection the reaction reaches the exponential phase (Phase II) which is the region where the Cq ...

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  • Life Science Equipment and Instruments Fisher Scientific

    Life Science Equipment and Instruments Thermo Scientific™ Owl™ Casting Dams Block off a portion of the gel tray and allow shorter gels or gradient gels to be cast and run with the high-quality aluminum Thermo Scientific™ Owl™ Casting Dams.

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  • Introduction to Real-Time PCR Primer Design

    These advantages of the fluorescence based real time PCR technique have completely revolutionized the approach to PCR-based quantification of DNA and RNA. ... SYBR Green remains stable under PCR conditions and the optical filter of the thermocycler can be affixed to harmonize the excitation and emission wavelengths. ... Specific detection of ...

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  • Fluorescence Applications Chroma Technology Corp

    Likewise emission filters should be chosen to best separate the donor /acceptor emission spectra. For example in some cases it may be best to red-shift the acceptor bandpass to minimize the detection of donor fluorescence emission while in other cases this will limit detection of the acceptor emission too much and will be counter-productive.

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  • Microplate Readers Fisher Scientific

    RNAi Oligos Assays Gene Editing & Gene Synthesis Tools Oligos Tools. Eurofins MWG Operon Oligos Tool

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  • Optical System for Multi-Channel Real-Time PCR Detection

    Optical module for multi-channel real-time PCR detection system is designed and implemented. By using hyperspectral band-pass filter integrated on CMOS photo sensor Multichannel optical fluorescence excitation and detection system are realized in experiments.

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  • qPCR Machines and Real-time PCR Platforms

    The most important aspects of current real time quantitative PCR strategies instrumentation and software and the application of qPCR technology in various areas of applied microbiology. Topics covered include instrumentation fluorescent chemistries quantification strategies data analysis software environmental microbiology water microbiology food microbiology gene expression studies ...

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  • Rotor-Gene Q - QIAGEN Online Shop

    Detection is similarly uniform. When each tube aligns with the detection optics the sample is illuminated and the fluorescent signal is rapidly collected from a single short optical pathway. This thermal and optical uniformity results in sensitive precise and fast real-time PCR analysis (see figure Precise real-time PCR analysis ). It also ...

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  • Design and Functional Attributes of the Streck Zulu RT

    detection filters; each filter is optimized for its proper function. These custom high-performance optical filters are designed to be compatible with most common fluorophores (Table 1). A separate set of optical filters block stray LED light and transmit the sample fluorescence emission …

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  • OEM Capability - Your Optical Filters Partner for ...

    This is an indication about the filters specification we could produce. It doesnt mean the standard is the same as the specification listed above. Optolong welcomes any OEM requirement about optical filter interference filter fluorescence filters astronomical filter.

    Get Price
  • Integrated wavelength-selective optical waveguides for ...

    Through the detection of a BK virus polymerase chain reaction (PCR) product we demonstrate that the dye-doped PDMS system is an order of magnitude more sensitive than a similar undoped system (SNR: 138 vs. 9) without the use of any external optical filters at the detector.

    Get Price
  • Supporting Information for: Visible Fluorescence Detection ...

    Visible Fluorescence Detection of Type III Protein Secretion ... The SopE2 promoter region and coding sequence was amplified by PCR from S. typhimurium strain IR715 a ... 488 nm and the emission was collected through a BP 505-550 filter. TOPRO-3 was excited with a HeNe laser at 633 nm and the emission was collected through a LP 650 ...

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  • Staphylococcus aureus Streptococcus agalactiae

    Each qPCR reaction contains four sets of primers and fluorescence probes for specific detection of ... • Pipettes and sterile filter tips ... ROX (585nm-610nm) CY5 (635nm-665nm). 5 4. PROTOCOL DNA purification 1. Spin a 96 Deep Well Plate 1 min at 1000 g and remove seal. Add 0.5 ml milk sample to

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  • LightCycler 480 Advanced Techniques

    reassign the Fluorescence in each channel to the appropriate dye • The net result is detection of only one dye signal in each channel. A CC object can only be applied to experiments that were run on the same LightCycler 480 Instrument it was created on.

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  • Shunting microfluidic PCR device for rapid bacterial detection

    1. Introduction. PCR has revolutionised the analysis of nucleic acids. There is an increasing trend in the use of microfluidic devices for point-of-care testing [].Robust portable microfluidic PCR systems for pathogen detection for point-of-care (PoC) food control clinical or environmental applications could supplement or replace expensive bench top laboratory apparatus [].

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  • CFX96 Touch™ Real-Time PCR Detection Systems from Bio-Rad ...

    Use the CFX96 Touch Real-Time PCR Detection System for precise reproducible real-time PCR. ... the fast scan option reads single-channel fluorescence in all 96 wells in just 3 seconds. A channel with an LED filter–photodiode combination is dedicated to FRET singleplex experiments.

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  • OSA Multivariate optical computing using a digital ...

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  • MODULE 5: SINGLE MOLECULE FLUORESCENCE

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  • The Importance of Flexibility in Spectrophotometry

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  • Metal-enhanced fluorescence of an organic fluorophore ...

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    the SPC by the BD MAX System. The VIM and IMP genes are combined in one optical channel of the BD MAX system; all other genes have a separate optical channel. When the probes are in their native state the fluorescence of the fluorophore is quenched due to its proximity to the quencher. However in the presence of target DNA the probes ...

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